In the present study simple, precise, rapid, sensitive, accurate and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated for sertraline hydrochloride. The chromatographic separation was carried out by using reverse phase stainless steel column of (4.5 mm × 25 cm) Intersil® C18 column with 5 μm (particles packing) held at 25°C. The mobile phase consisted of acetonitrile: sodium phosphate buffer of pH 4.7 (88:12 v/v), with a flow rate of 1.0 ml/ min, UV detection at 271 nm and injection volume 20 μl was maintained. The retention time was found to be 3.628 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to International Conference on Harmonization (ICH) guidelines. The linearity was observed in the range of 50-300 μg/ml with correlation coefficient, r = 0.996.
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